A fielddeployable, chemical ionization timeofflight mass spectrometer article pdf available in atmospheric measurement techniques 47. Chemical ionization mass spectrometry, 2nd edition will be a valuable reference for anyone interested in mass spectrometry and gaseous ion chemistry in general. Atmospheric pressure chemical ionization apci is an ionization method used in mass spectrometry which utilizes gasphase ionmolecule reactions at atmospheric pressure 10 5 pa, commonly coupled with highperformance liquid chromatography hplc. When the ei or ci source is replaced by an esi source, all of the analytical processes are conducted. The chemical ionization source is being used less and less in mass spectrometry compared to new modern ionization techniques such as maldi or electrospray 3. An electron impact ei or a chemical ionization ci ion source can be used to operate the mass spectrometer. Chemical ionization mass spectrometry of nitroaromatic vapors. Modern mass spectrometry the instrumentation and applications in diverse fields mass spectrometry has played a pivotal role in a variety of scientific disciplines. Double bond localization in unsaturated fatty acid methyl. Atmospheric pressure chemical ionization apci apci is an ionization method used in mass spectrometry commonly lcms which utilizes gasphase ionmolecule reactions at atmospheric pressure. Quantitative analysis of triglycerides using atmospheric pressure chemical ionizationmass spectrometry article pdf available in lipids 319. Fundamentals of contemporary mass spectrometry wiley. Ci is an indirect process involving an intermediate chemical agent.
One general problem with ci is the generally lower intensity of peaks observed compared to ei. Combination of gas chromatography and chemical ionization mass spectrometry. Chemical ionization mass spectrometry crc press book. Electrospray ionization for mass spectrometry of large. Today it is an integral part of proteomics and drug discovery process.
Chemical ionization mass spectrometry cims is a technique for forming ions of the compound of interest analyte, a by ionmolecule reactions from reactant ions of a reagent gas that is generally present in a much greater abundance than the analyte. Molecules can be ionized by transfer of an electron, a proton, or other charged species, and either positive or negative ions can result. Chemical ionization an overview sciencedirect topics. Fundamentals of contemporary mass spectrometry gives readers a concise and authoritative overview of modern mass. Ambient ionization techniques allow complex chemical samples to be analyzed in their native state with minimal sample preparation.
Imaging mass spectrometry based on maldi and sims secondary ion mass spectrometry has become a powerful technique for analyzing histological sections of biological tissues 17, 18. Chemical ionization mass spectra of classes of compounds are discussed in detail to illustrate the influence of different functional groups on the spectra observed. Pdf chemical ionization mass spectrometer cims for ambient. Ionization chemical ionization ci ei is a direct energy transfer process with electron kinetic energy deposited directly into an analyte molecule. Data system mass detector analyzer vacuum system source inlet region more precisely mass spectrometry determines the mass of a molecule. From the time of the second edition published in 2001 until now, much progress has. Alternate or simultaneous electron impact chemical ionization mass spectrometry of gas chromatographic effluent. Molecular differentiation of five cinnamomum camphora. Electrospray ionization mass spectrometry is a desorption ionization method. A reagent gas is introduced separately and continuously at a higher.
Apci is a soft ionization method similar to chemical ionization where primary ions are produced on a solvent. It is an ionization method that is similar to chemical ionization commonly used in gcms where corona discharges on a solvent spray produce primary. Fundamental aspects of organic mass spectrometry or metastable ions, out of. We used desorption electrospray ionization mass spectrometry in an. The latest edition of a highly successful textbook, mass spectrometry, third edition provides students with a complete overview of the principles, theories and key applications of modern mass spectrometry. Reagent gas molecules are ionized by electron ionization, which subsequently react with analyte molecules in the gas phase in order to achieve ionization. Ionization techniques the ionization takes place in the ion source. Chemical ionization mass spectrometry, second edition. The mass to charge ratio mz is used to describe ions observed in mass spectrometry. An extension of the technique of chemical ionization mass spectrometry utilizing moist n2 or air as the reactant gas has been studied. Sharad medhe department of chemistry, educational institute, dayalbagh, agra282005, india. Another problem is that no large spectral library exist as the nist db for ei spectra which makes identification a very unpleasant experience. Conventionally, analyte molecules exiting a gc or a direct insertion probe are ionized in the electron impact ei or chemical ionization ci source in a vacuum 8, 19, 20.
To an extent greater than has previously been possible with the more familiar soft ionization methods, this technique makes the power and elegance of mass spectrometric analysis applicable to the large and. Electrospray ionization has recently emerged as a powerful technique for producing intact ions in vacuo from large and complex species in solution. This technique is a branch of gaseous ionmolecule chemistry. Amines are of interest to atmospheric chemistry as they may be important gasphase precursors for secondary aerosol.
A novel method for the quantitative determination of chlorinated paraffins cps was developed using bromideanion attachment atmosphericpressure chemical ionization mass spectrometry apcims. Chemical aspects of the extractive methods of ambient. Pdf this study describes a chemical ionization mass spectrometer cims for fast response, insitu measurements for gas phase ammonia. Gas chromatographmass spectrometry, mass spectrometry keywords. This was first introduced by burnaby munson and frank h. However, these techniques require that the sample be confined to the highvacuum region of the instrument, severely limiting any further chemical or. A crossref search on chemical ionization mass spectrometry. Latent fingerprints lfps potentially contain more forensic information than the simple identification of the subject. By convention, m is the numerical value for the mass of the ion and z is the numerical value for the charge of the ion. Principle of operation chemical ionization ci is a form of high pressure mass spectrometry 0. The reactions produced in the ci source are numerous and complicated, but the ci source offers a good understanding of the chemical reactions in the gas phase that do not occur in solution. Electron impact ionization delivers the highest energy to the analytes leading to hard ionization and causes a large amount of molecular fragmentation.
Mass spectrometry ms is mainly involved in analysis of masstocharge ratio mz and large quantity of ions traveling at high speed in vacuum. The reactant ions are generally produced by electron ionization ei of the reagent gas. In pci, the ion source is filled with a reagent gas which is. Chemical ionization mass spectrometry 2nd edition alex. Chemical ionization ci is a soft ionization technique used in mass spectrometry.
This is particularly true in positive chemical ionization pci. Smith, in encyclopedia of atmospheric sciences second edition, 2015. General principles on mass spectrometry sample introduction ionization of sample in the ion source separation of ions by mass analyzer detector computer system resulting chromatogram 4. Pdf chemical ionisation mass spectrometry for the measurement. Spectroscopic methods with nanoscale lateral resolution are becoming essential in the fields of physics, chemistry, geology, biology, and materials science. Soybean oil triacylglycerol analysis by reversedphase. Chemical ionization mass spectrometry is a selective and highly sensitive method for molecular. The quasiequilibrium theory qet of mass spectra is a theoretical approach to.
Ionization methods in organic mass spectrometry jeol usa inc. Molecular differentiation of five cinnamomum camphora chemotypes using desorption atmospheric pressure chemical ionization mass spectrometry of raw leaves. Electron impact mass spectrometry 70 ev 1614 kcalmol contrast with energy from ir 110 kcalmol or nmr 0. Before the sample can be mass analyzed, it must be ionized in the ion source. Brinded ka, tiller pr, lane sj 1993 triton x100 as a reference compound for ammonia highresolution chemical ionization mass spectrometry and as a tuning and calibration compound for thermospray. Chemical information can also be useful in resolving overlapping lfps from different individuals. Latent fingerprint chemical imaging by mass spectrometry. Chemical reactivity assessment using reactive paper spray ionization mass spectrometry. This brings the obvious advantages of simplicity, speed, and versatility to mass spectrometry. Chemical ionization ci relies on gasphase chemical reactions that take place between the analyte of interest and ions generated from a reagent gas skoog and leary, 1992a,b,c,d. Determination of chlorinated paraffins by bromideanion. The katritzky reaction xin yan department of chemistry, purdue university, 560 oval drive, west lafayette, in 47907.
Tipenhanced ablation and ionization mass spectrometry for. However, the lateral resolution of laserbased mass spectrometry imaging msi techniques has so far been limited to the microscale. This report presents the development of tipenhanced ablation and ionization. The contents of the solvent spray, the gas flow rate, the amount of applied voltage, the spray angle and the ion uptake angle, as well as the various distances in aligning the spray, sample and mass spectrometer are all variables which can be studied to achieve an optimal mass spectrum for a particular type of sample. Coupling and ionization in miniature mass spectrometry. Desorption ionization methods can be performed on solid or liquidsamples, and allows for the sample to be nonvolatile or thermally unstable.
Electron impact or chemical ionization for mass spectrometry. Chemical ionization uses gas phase ionmolecule reactions within the vacuum of the mass spectrometer to produce ions from the sample molecule. All instrumental aspects of mass spectrometry are clearly and concisely described. Ci differs from what we have encountered in mass spectrometry so far because. Chemical ionization ci is the first of the soft ionization methods we are going to discuss. Desorption electrospray ionization desi, for example, is used in chemical imaging for tumor margin diagnosis.
The use of esi to postionize analyte molecules can be traced back to the gcesi technique reported in 1998. Chemical reactivity assessment using reactive paper spray. Investigation of polytertiary alkylamines using chemical ionization mass spectrometry. Fatty acid methyl esters fame, polyunsaturated fatty acids, covalent adduct chemical ionization, structural analysis of fatty acids, environment, food and beverages, gcmstq8050 nx language.
1412 151 1478 672 768 1141 965 1532 1105 766 665 1413 750 175 504 293 806 1382 1499 1337 52 951 123 141 658 1225 13 1614 313 67 94 569 553 14 389 762 1387 968 1356